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Future, we taken care of cells with different concentrations of NAD+. When extra for twenty-four h at eighty µM in HeLa and 400 µM in U2OS cells, NAD+ impaired the incorporation of EdU (Fig. 2a and Extended Details Fig. 1k,l). Furthermore, 16 µM of NAD+ was ample to cut back fork pace by ~forty% in HeLa cells with out impacting the overa